alexa Genotyping Of Cryptosporidium Species Isolated From Human Stool Using PCR-RFLP According To 18s RRNA Gene (Shahrekord, Iran)
ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
Open Access

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4th International Congress on Infectious Diseases
May 11-12, 2017 Barcelona, Spain

Bahman Khalili, Rahman Abdizadeh and Elham Moieni Sangjooie
Shahrekord University, Iran
Posters & Accepted Abstracts: J Infect Dis Ther
DOI: 10.4172/2332-0877-C1-024
Abstract
Background & Aim: Cryptosporidium is an obligatory intracellular-extra-cytoplasmic parasite and also one of the most important pathogens causing diarrhea in human and animals. The aim of this study was investigation of Cryptosporidium prevalence in Shahrekord and also determining the most common species causing diarrhea in the district. Methods: A total 1893 fecal specimens were collected from patients with acute or chronic diarrhea. Samples were stained with modified Ziehl-Neelsen method. Positive samples were collected for Cryptosporidium examined by RLFP PCR to detect Cryptosporidium species. Results: In microscopic examination out of 1893 diarrheic fecal specimens, 20 isolates (1.05%) were positive for Cryptosporidium. PCR procedure confirmed 17 cases out of 20 positive samples (0.89%), and three positive specimens by Ziehl-Neelsen staining were negative in PCR procedure. The result of RFLP PCR showed that all positive samples were diagnosed as C. parvum. Conclusion: The results of this study revealed that the frequency of Cryptosporidiosis infection has a higher rate among children. According to type of the parasite isolated in the study (C. parvum), we have to inform local health policy maker to prepare appropriate control programs for this zoonotic parasite.
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