alexa Global Gene Expression In Escherichia Coli, Isolated From The Diseased Ocular Surface Of The Human Eye With A Potential To Form Biofilm
ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

6th Euro-Global Conference on Infectious Diseases
September 07-09, 2017 | Paris, France

Ranjith Konduri
LV Prasad Eye Institute, Hyderabad, India
ScientificTracks Abstracts: J Infect Dis Ther
DOI: 10.4172/2332-0877-C1-032
Abstract
The surface of the eye is colonized by several bacteria, which survive as resident or transient commensals. But following trauma or under immuno-compromised conditions these commensals cause infection of the eye (such as keratitis, endophthalmitis, orbital cellulitis etc.) often leading to loss of vision. Normally the infection is resolved following treatment with antibacterial agents. However, over the years many of these organisms have become resistant to drugs due to excessive and indiscriminate use of drugs. Resistance to drugs may be due to biofilm formation which makes the bacteria impervious to antibiotics. In the present study ocular E. coli from patients with ocular infectious disease is used as a model system and was screened for their ability to form biofilm, antibiotic susceptibility. In addition, to understand the molecular mechanisms underlying the biofilm formation and resistance to antibiotics in biofilm phase we used DNA microarray. Ten of twelve ocular E. coli isolates were resistant to at least one or more of nine antibiotics tested and majority of isolates were positive for biofilm formation. E. coli L-1216/2010 is best biofilm forming isolate confirmed by confocal and scanning microscopy. Further E. coli in the biofilm phase was 100 times more resistant to antibiotics tested compared to planktonic phase. DNA microarray analysis could differentiate E. coli biofilm forming cells from non-biofilm forming planktonic cells. It was noted that 30% (10.5% up and 19.5% down-regulated) genes were differentially regulated in the sessile biofilm forming cells compared to the non-biofilm cells. Genes encoding cell adhesion genes, extracellular matrix are upregulated in biofilm phase. In addition, some of the up-regulated genes encoding antimicrobial efflux virulence, toxin production, and other metabolites are known to affect the antibiotic susceptibility of planktonic. These genes serve as potential targets for hacking biofilms. This is the first study on whole genome expression of ocular E. coli isolates with a potential to form biofilm. Study on native pathogenic ocular isolates for biofilm and antibiotic susceptibility is more relevant than type strains which do not necessarily mimic native isolates.
Biography

Ranjith Konduri has done his MSc from the Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry. He has qualified GATE with percentile of 99.7%. Currently he has registered for PhD with infectious diseases as area of interest. The work involves the identification and functional characterization of genes involved in antibiotic resistance and biofilm formation. It aims to identify and understand various molecular mechanisms involved in cell adhesion for the dispersal of the biofilm. Very recently he has published a research article on Biofilm in Journal Gut Pathogens.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords