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Synthesis of gold nanoparticles by biological means leads to, eco-friendly, non-toxic, quicker synthesis and is commonly called
as green method. In this study, plant leaf extract was prepared for gold nanoparticle synthesis. Physicochemical factors were
optimized with respect to pH, temperature, time of incubation and gold salt concentrations for synthesis of gold nanoparticles.
The optimum gold salt was 0.5 mM, pH 7.0, time 50 minutes and temperature 500C for synthesis of gold nanoparticles. Gold nanoparticles were characterized by using UV/Vis spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive spectroscopy (EDS) and X-ray diffraction analysis (XRD). TEM studies revealed that
spherical nanoparticles were formed with a size in the range of 10-20 nm. Using these Au nanoparticles, lipase enzyme was
successfully immobilized on sodium alumino silicate. Immobilized enzyme was stable and showed more activity as compared to
Rasika Kulkarni is M.Sc. student at Department of Microbiology, University of Pune, Maharshtra, India.
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