Journal of Biotechnology & Biomaterials
Open Access

Abstract

In the present study, the larvicidal potential of silver nanoparticles (AgNPs) synthesized from Annona muricata plant leaf extract against fourth instar larvae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti were determined. Various concentrations of synthesized AgNPs (10, 20, 30, 40 and 50 ppm) and the aqueous leaf extract (100, 200, 300, 400, and 500 ppm) were tested. AgNPs were characterized by UV-Vis spectrum, Fourier transform infrared spectroscopy, Particle size analyser, X-ray diffraction, High Resonance Transmission electron microscopy and energy-dispersive X-ray spectroscopy analysis. Larvae were exposed to different concentrations of aqueous and AgNPs for 24 and 48 hours. The maximum mortality was noticed in AgNPs than aqueous leaf extract of Annona muricata against Anopheles stephensi had LC50 values of 24 hours 37.70, 546.7, and 48 hours 25.47, 458.2 ppm and the LC90 values of 24 hours 73.67, 937.0 and 48 hours 53.18, 852.0 ppm. Culex quinquefasciatus had LC50 values of 24 hours 31.29, 516.2 and 48 hours 21.10, 442.3 ppm and the LC90 values of 24 hours 59.54, 936.6 and 48 hours 44.34, 807.2 ppm. Aedes aegypti had LC50 values of 24 hours 20.65, 618.4 and 48 hours 6.35, 349.1 and the LC90 values of 24 hours 45.58, 1240.6 and 48 hours 7.99, 703.7 ppm, respectively and the 100% mortality was observed in AgNPs against Aedes aegypti after 24 hours in all the concentrations. The antimicrobial activity of AgNPs was investigated against gram positive and gram negative bacteria and fungi. The antibacterial activity of AgNPs was more effective than commercial antibiotics. This study also focused the potential application of AgNPs to target the free radicals scavenging in vitro. Further, we have explored the cytotoxicity effect against human breast cancer cells. These results revealed that the use of Annona muricata synthesized AgNPs can lead a pathway to develop effective biological applications.

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