Journal of Biotechnology & Biomaterials
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This investigation was conducted to study the effects of copper on growth and metabolism of
isolated from sewage water at El-Dare Treatment Plant. The alga was grown in BG-11 medium containing different cu
concentrations (0.0, 0.5, 5, 10, 50, 100, 150, 200 and 250μm Cu
). The test organism was left to grow for 12 days under
the various Cu levels. The effect of copper on the growth, photosynthetic pigments, protein metabolism (soluble, insoluble),
antioxidant compounds (phenolic, Proline) were determined. The cell number and dry weight were increased highly
significantly up to 10 μm Cu
), then the cell number and dry weight reduced gradually by increasing the doses of the copper.
The concentration of photosynthetically active pigment peaked up to 10 μm Cu
), Then they remained more or less unchanged
up to 50 μm Cu
), after this it was reduced. The content of soluble carbohydrates decreased dramatically up to 10 μm cu+2, and
then it was increased smoothly as the cu concentration increased in culture media. Cu
Stress induced a significant changes
in the soluble protein up to the level of 10 μm Cu
, then a high significant accumulation was obtained which peaked at the
highest concentration of Cu+2 (250 μm). Theinsoluble and total proteins remained more or less unchanged at the all Cu
concentration used. Aminoacids content accumulated progressively and irregularly by Cu
treatment. While proline content
decreased by about 65.6% at the level of 10μm Cu+2concentration. It is onother hand increased by 261.7% at the level of 250μm
in relation to control value. Phenolic compounds seemed to be not affected by copper treatment.
Magdy A Abo- Gharbia is an Assistant professor in Faculty of Science, Sohag University, Egypt. He obtained his PhD in Microbiology, Dundee University, Scotland
(1989). He has more than 5 publications in various repute journals.
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