Journal of Biotechnology & Biomaterials
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Retinoblastoma is the most common primary intraocular childhood malignancy. Earlier studies have suggested an association
of HPV with sporadic retinoblastoma, indicating that the virus may have a role in the development of these tumors. Marked
variation (0-40%) of HPV positivity in retinoblastoma reported could be due to geographical factors or differences in detection
methods used. In this study, presence of HPV in retinoblastoma was detected by various diagnostic techniques. These included
PCR (using both MYO9/11 and PGMYO9/11 primers), In-situ hybridization (ISH) and Immunohistochemistry (IHC). These
techniques were further validated by reverse hybridization (linear array) which detects 37 genotypes of HPV.
Of the 20 retinoblastoma cases recruited, (16 unilateral and 4 bilateral, mean age of 4 years and M: F ratio 2.3:1), 18 were
poorly differentiated (PD) and 2 well differentiated. Optic nerve and choroidal invasion was seen in 15 cases. HPV was detected
in 40% (8 cases) using MYO9/11 primers. However, none of the cases were positive for HPV by PGMYO9/11 primers. Of the 8
cases positive by MYO9/11 primers, 6 were positive by IHC and 3 by ISH. However, on validation by linear array none of the 37
genotypes were identified in any of the retinoblastoma cases.
The results of our study show that although PCR is a sensitive technique for HPV detection, PGMYO9/11 primers were
found to be more specific then degenerate MYO9/11 primers.
Ms. Sheetal Chauhan has completed her Ph.D work entitled ?Molecular studies and expression of cell cycle regulatory proteins in Ocular Surface
Squamous Neoplasia? from the department of ocular pathology, Dr. R.P Centre, All India Institute of Medical Sciences, New Delhi, India.. Her work
in ocular oncology has been published in reputed international journas and national/international conferences.
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