Journal of Biotechnology & Biomaterials
Open Access

Abstract

Hyaluronic acid (HA), a glycosaminoglycan with high molecular weight, has been reported to promote cell proliferation and serves as an important extracellular matrix component. The aim of this study was to in vitro investigate whether HA is able to reduce reactive oxygen species (ROS)-induced heart ischemia reperfusion injury and activate the cardiomyocyte?s damage surveillance systems. Accordingly, rattuscardiomyocyte line, H9C2, was treated with H 2 O 2 as a heart ischemia reperfusion model followed by incubation with low molecular weight hyaluronan (LMW-HA,100 kDa) or high molecular weight hyaluronan (HMW-HA,1000 kDa) and proteomic analysis was performed to investigate the physiologic protection of HA in H 2 O 2 -induced ischemia reperfusion in cardiomyocyte. The data demonstrated that HA treatment does protect cardiomyocyte in the ROS-induced ischemia reperfusion model and the molecular weight of HA is a crucial factor. HMW-HA has been shown to significantly facilitate cell migration and wound healing via cytoskeletal rearrangement. Additionally, 2D-DIGE combined MALDI-TOF/TOF analysis showed that HMW-HA might modulate biosynthetic pathways, cell migration, cell outgrowth and protein folding to stimulate wound healing as well as prevent these ischemia reperfusion-damaged cardiomyocytes from cell death. It is reported for the first time the cell repair mechanismof HMW-HA against ischemia reperfusion-damage in cardiomyocytes based on cell biology and proteomic analysis.

Biography

Hong-Lin Chan is head of the National Tsing-Hua University (Taiwan) for Quantitative Proteomics Group and has more than 10 years of experience in proteomic method development and application. He received his PhD degree from University College, University of London in 2005. After 2 year?s post-doctoral training in the Wolfson Institute for Biomedical Sciences, he took the current professorship from National Tsing-Hua University in Taiwan. His group is focused on: Serum biomarker discovery, characterising redox and UV stress responses in cell models, mechanisms of cellular signalling, proteomics based studies on breast cancer, prostate cancer and drug resistance formation.