Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Hyper Thermo Philic Phosphotriesterases/lactonases: Structure, Function And Applications | 17120
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Hyper thermo philic phosphotriesterases/lactonases: Structure, function and applications

5th World Congress on Biotechnology

Giuseppe Manco

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.027

Recently, a new family of microbial lactonases with promiscuous phosphotriesterase activity has been discovered, dubbed PTE-Like Lactonases (PLL) and ascribed to the amidohydrolase superfamily. Among members of this family are enzymes found in the Archaea: Sulfolobus solfataricus and Sulfolobus acidocaldarius , which show high thermophilicity and thermal resistance. From a biotechnological point of view enzymes endowed with phosphotriesterase (PTE) activity are attractive objects of study because are capable to hydrolyze the organophosphate phosphotriesters (OPs), a class of worldwide used synthetic compounds employed both as insecticides and chemical warfare agents. From the viewpoint of basic research, studies of catalytic promiscuity offer clues to understand the natural evolution of enzymes and to translate this knowledge into in vitro adaptation of catalysts to specific human needs. Thermostable enzymes able to hydrolyze natural lactones and promiscuously OPs are currently considered good candidates for the set-up of efficient anti-microbial and detoxification tools respectively. The author will report their attempts to follow these two paths. It will be shown that thermostable PLLs are effective in interfering with quorum-sensing mediated expression of virulence factors in vitro and in vivo . On the other hand successful approaches of increasing PTE activity by molecular evolution in archaeal enzymes will also be reported.
Giuseppe Manco is Senior Staff Researcher (1st Researcher) and Group Leader at Institute of Protein Biochemistry, National Research Council (CNR) of Italy. He graduated in Biological Sciences at the University Federico II of Naples in 1987 and in 1989 he got the Diploma to exert the Profession of Biologist at the same University. Then he moved to CNR working as recipient of several fellowships first at the International Institute of Genetics and Biophysics and then at the Institute of Protein Biochemistry and Enzymology. In 1995 he was for a short period at NIH, Bethesda, USA in the Lab of E. Stadtman and R. Levine as visiting scientist. Starting from 1994 until 1997 he was hired as Researcher at the Institute of Protein Biochemistry and Enzymology with a non-permanent position, which became permanent in October 1997. In 2001 he became Senior Staff Researcher (1st Researcher) in the same Institute. From 2003 to 2009 he was elected member of the Institute Committee. Since 1998 Dr Manco started leading his independent research group and as such had the responsibility of Regional, National and International projects. At the moment he is coordinating a large Italian project from the Italian Ministry of Research (MIUR) in the field of Security and Environment. He is member of the CNBRE group of SERIT (Security Research in Italy) a consortium between CNR and Finmeccanica; member of the Group of Technological Transfer of the Regional District in Biotechnology ?Campania Biosciences?; member of the Italian Society of Microbiology. He is co-author of more than 100 papers in the field of biochemistry and molecular biology.