Identification And Characterization Of Bio-remediating Bacterial Isolates From Soil And Water | 4658
Journal of Biotechnology & Biomaterials
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Bioplastics are natural biopolymers have certain advantages over petroleum-derived plastic that do not cause toxic effects in the host. These are synthesized and catabolized by various microorganisms as a by-product. The world is currently using approx 140 million tons of plastics per annum consuming approximately 150 million tons of fossil fuels for processing of these plastics. Substitution of such a big demand is difficult for coming eras therefore bi-plastic provide a base for this industry as eco-friendly plastic. These biopolymers accumulate as storage materials in microbial cells under stress conditions. The most widely
produced microbial bioplastics are polyhydroxyalkanoates (PHAs) and their derivatives. Ralstonia eutropha H16 is a gram-
negative, rod-shaped, and facultatively chemolithoautotrophic hydrogen-oxidizing bacterium that serves as a model organism
for polyhydroxyalkanoate (PHA) metabolism. Mainly found in soil and water and this bacterium has great ability to use in
bioremediation, as it is able to degrade a great number of chlorinated aromatic (chloroaromatic) compounds and chemically related pollutants. Therefore, in the study the different isolates of bacteria strains were isolated from different soil and water sources. To identify these bacterial strains a 16S rDNA gene based approach was applied. Genomic DNA isolated using Gen Elute
Bacterial Genomic Kit (Sigma). PCR conditions optimised to amplify 16S rDNA by using universal known primers set of rDNA at different annealing temperature in a gradient PCR. The PCR amplicon sequenced commercially and phylogentic analysis done by sequence homology search with the known sequences from the gene bank and taxonomic position of the new isolates was established.
Manorama Singh has completed his M.Tech in Biotechnology from School of Biotechnology, Rajiv Gandhi Prodaugiki Viswavidhayalaya, Bhopal,
Madhya Pradesh. Presently she is working as Junior Research Fellow in the Division of Veterinary Biotechnology, Indian Veterinary Research
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