Journal of Biotechnology & Biomaterials
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Th e dune grass Leymus mollis (Triticeae; Poaceae,
genus Leymus) is a wild relative of wheat and grows
mainly along sea coasts and in inland dry areas (Kishii et
al. 2003). Th e tolerance of the dune grass Leymus mollis
(Triticeae; Poaceae) to various biotic and abiotic stresses
makes it very useful genetic resource for wheat breeding.
Wide-hybridization between L. mollis and wheat
allowed the introduction of Leymus chromosomes into
wheat genetic background and facilitated the integration
of useful traits into wheat. However, the genetic bases
controlling L. mollis physiological tolerance to multiple
environmental stresses remain largely unexplored.
Using suppression subtractive hybridization, we
identifi ed 116 drought stress responsive genes from L.
mollis and confi rmed their diff erentially expression by
drought. Th ese gene were categorized into 13 functional
category including cell defense and stress response,
transcriptional regulation, signal transduction,
biosynthesis of compatible solutes and metabolism of
cell walls in response to drought stress. Analyses of the
expression patterns in response to drought stress and
abscisic acid treatment by northern blot and RT-PCR
were validated for selected genes. Furthermore, some
selected genes were shown to be up-regulated under
salt stress. Th e genes identifi ed in this study represents
valuable source as expressed sequence tags (ESTs)
for analysis and identifi cation of alien chromosomes
introduced into wheat. Furthermore, being highly
conserved, genetically associated with drought tolerance
and transferable to wheat, these ESTs provide signifi cant
tools for the development of EST-derived markers and
for assaying variations in the transcribed parts of the
complex and highly redundant wheat genome.
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