Identification Of Triticale- Rye Hybrids With ISSR-PCR Markers Application | 17050
Journal of Biotechnology & Biomaterials
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The F1 hybrids between maintainers of the cms-
system in triticale and maintainers of the cms-Pampa
system in rye were obtained and their hybrid origin was verified. The F1 hybrids were selfing and maintaining ability effect
of F2 individuals in triticale cms lines with
cytoplasm, were tested. After pollinating over seven thousands flowers
of triticale with rye pollen, ten hybrid grains were obtained (0.14%). Their hybrid origin was verified by PCR using ISSR (Inter
Simple Sequence Repeats) primers. Our sets primers produced 24 to 49 amplification fragments of different lengths. Among
them, several fragments were monomorphic, i.e., common to maintainers of triticale, maintainers of rye and their F1 hybrids.
In most primer-genotype combinations from one to four fragments were specific to the rye maintainers and the F1 hybrids.
These data provide evidence for the hybrid character of the F1 plants. These fragments were not observed in female triticale
maintainers. It is worth noting that in most of the studied hybrids more PCR fragments were obtained in female parents
compare to male parents, this is most noticeable in the case of primer ISSR03. The most informative of the primers was ISSR
03 that generated seven polymorphic fragments that were present in male parents and the hybrids, and 18 fragments that were
present in female parents and the hybrid. Fourteen F2 individuals (7.8%), as well as their S1 and progeny, generated stable male
sterility in F1, BC1 and BC2 generations possessing
Tomasz Warzecha has completed his PhD in 2001 from Agricultural University in Krak
w, Poland. He has participated at the International Postgraduate Course
on Biotechnology in Agriculture, Plants and Microorganisms at the Hebrew University of Jerusalem. Additionally completed the Pedagogical Studium majored in
Biology and Chemistry at Jagiellonian University in Krakow, Poland. He has worked in a project focused to examine natural variation in the recombination pathways
in maize at the Department of Plant Breeding and Genetics at Cornell University, Ithaca, USA. At present he is Research Associate at the Department of Plant
Breeding and Seed Science in Krakow.
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