alexa

GET THE APP

Identification Of Uropathogens Using Faco S Media: A Comparative Study With Other Conventional Media | 49581
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

6th International Conference on Vaccines and Immunology

Geneva, Switzerland

3rd European Congress on Virology

Geneva, Switzerland
Google Scholar citation report
Citations : 1600

Journal of Biotechnology & Biomaterials received 1600 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Identification of Uropathogens using Faco s Media: A comparative study with other conventional media

11th World Congress on Biotechnology and Biotech Industries Meet

Festus Chukwuemeka Onwuliri, Onwuliri E A and Mgbachi N P

Federal University Lafia, Nigeria University of Jos, Nigeria

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.052

Abstract
Faco’smedium is a novel, differential culture medium obtained from the mixture of extracts of Acha (Digitariaexilis and Digitariaiburua) and pooled human urine that is claimed to facilitate the isolation and presumptive identification of some clinical important bacterial isolates from clinical samples. The microbiological performance and efficacy of Faco’s medium was compared to that of blood and MacConkey agar for the isolation and presumptive identification of bacteria responsible for urinary tract infections. This study included consecutively collected midstream and/or catheter-catch urine samples obtained from patients. All urine samples were inoculated on Blood agar, MacConkey agar, and Faco’s mediummedium and incubated overnight aerobically at 370 C and examined. Of the 2700 urine samples tested, 71.3% produced significant growth on the test media and about 14 potentially significant organisms were recovered on at least one of the three media. 743(95.8%) were uni-microbial, 32(4.12%) were poly microbial. Faco’s mediummedium succeeded in detecting all the urine pathogens that were detected by the reference media, including Gram-negative bacilli, Staphylococci, Streptococci and Yeasts. Colony colour and morphology on Faco’s medium agar accurately differentiated Escherichia coli, Proteus species, Klebsiella, Pseudomonas and Acinetobacterspp. The result suggests that Faco’s medium agar media can be used as a single medium for the isolation of uropathogens. Presumptive identification of isolates is time consuming and requires a great deal of experience when using traditional media. On Faco’s medium it is easier and will improve the quality of urine culture by contributing to a more uniform interpretation of Uropathogens on culture media. The performance of Faco’s medium was also determined using bacterial growth curve and the result obtained showed that there was significant increase in the performance of Faco’s media compared to that of Nutrient Broth. The result obtained also showed that there was significant difference (P<0.05) between the growths of E. coli when grown in Faco’s media containing two different substrates. Growth in medium containing Nitrophenyl-B-Dgalactoside was better than the growth of E. coli in the medium containing glucose. The activity of the enzyme B-galactosidase induced was then determined. The implications of the results are discussed.
Biography

Festus ChukwuemekaOnwuliri has obtained his PhD from University of Jos, Nigeria. He has completed his BSc, MSc and AIML from the University of Nigeria Nsukka, University of Jos and Medical Laboratory College Vom, Nigeria respectively. He has acquired a wide range of administrative experience. He was the Head of Department of Plant Science and Technology, University of Jos and is the Director of Victory Medical Laboratory Service, Nigeria. He has published about 65 papers in both national and international journals. He has served in several committees, both ad-hoc and statutory, within the University of Jos and other Universities in Nigeria. He has several memberships including Association of Medical Laboratory Scientists of Nigeria, Nigerian Society for Microbiology, Nigerian Mycological Society, Botanical Society of Nigeriaand Nigerian Society for Parasitology, Biotechnology Society of Nigeria and International Biotechnology.

Email: [email protected]

Top