Immobilization And Characterization Of Inulinase From Ulocladium Atrum On Nonwoven Fabrics | 33969
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

2nd International Conference on Biotechnology and Healthcare

Auckland, New Zealand
Google scholar citation report
Citations : 1536

Journal of Biotechnology & Biomaterials received 1536 citations as per google scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Immobilization and characterization of inulinase from Ulocladium atrum on nonwoven fabrics

8th Euro Biotechnology Congress

Tarek M Mohamed1, Soad M Abu El-Souod1, Ehab M M Ali1, Mohammed O El-Badry2, Mai M El-Keiy1 and Aly Sayed Aly2,3

1Tanta University, Egypt 2National Research Centre, Egypt 3Shaqra University, KSA

ScientificTracks Abstracts: Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.037

Ulocladium atrum inulinase was immobilized on different composite membranes composed of chitosan/nonwoven fabrics. Km values of free and immobilized U. atrum inulinase on different composite membranes were calculated. Free and immobilized U. atrum inulinase had pH optima at 5.6 on polyester nonwoven fabric coated with 3% chitosan solution (PPNWF3) but immobilized U. atrum inulinase on polyester and polypropylene nonwoven fabrics coated with 1% chitosan solution had pH optimum 5. The enzyme had optimum temperature at 40 °C for immobilized enzyme on each of polyester and polypropylene composite membranes coated with 1% chitosan meanwhile it was 50 °C for free and immobilized enzyme on polypropylene nonwoven fabric coated with 3% chitosan solution. Inulinase was stable at 40 °C for free U. atrum inulinase but thermal stability of immobilized enzyme was detected up to 60 °C. Reusability of immobilized enzyme was from 38 to 42 cycles of reuse; the immobilized enzyme lost its activity completely. In conclusion, immobilized U. atrum inulinase was considerably more stable than the free enzyme and could be stored for extended periods.

Tarek M Mohamed has been a Professor of Biochemistry in Biochemistry Division, Chemistry Department, Tanta University, Tanta, Egypt since June 2008. He is a Reviewer for many journals. He shared in 12 national projects and supervised more than 20 MSc and PhD theses. He has 42 publications. He is interested in purification and characterization of enzymes, enzyme inhibitors, study related to enzyme engineering and biochemistry.

Email: [email protected]