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Immobilization Of Biomolecules From Aqueous Solution By Carbon Micro And Carbon Nanofiber | 4822
Journal of Biotechnology & Biomaterials
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Immobilization of biomolecules on carbon support has many applications, for example, in the development of biocatalyst and
biosensor, and wastewater treatment. In this study, the phenolic resin precursor based carbon activated fibers (ACFs) and
carbon nanofibers (CNFs) were used for the immobilization of a number of biomolecules such as glucose oxidase (GOx), bovine
serum albumin (BSA), and YQEH, a laboratory synthesized protein. These biomolecules used for the adsorption study had
different structures, isoelectric points (IP) and number of amino acids. CNFs were prepared by impregnating ACFs with nickel
nitrate using anionic SDS surfactant to achieve the mono dispersion of Ni ions in solutions followed by calcinations, reduction
and catalytic chemical vapor deposition (CVD) using benzene (C6H6) as a carbon-source. The metal particles on the tip of CNFs
were removed by ultra-sonication in acidic medium to open up the tips and provide increased number of sites for adsorption.
The prepared materials were directly used as adsorbents for biomolecules, with no further post-treatment required. The prepared
ACFs and CNFs were characterized by several analytical techniques such as atomic absorption spectroscopy (AAS), scanning
electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), BET surface area
and pores-size distribution (PSD) analyzers. The adsorption capacities of the prepared materials in this study were found to be
32 mg/g for GOx, 103 mg/g for BSA, and 38 mg/g for YQEH. These data reveal that the prepared ACFs and CNFs are potential
adsorbents for biomolecules.
Nishith Verma has PhD in chemical engineering from the University of Arizona, USA. After completing his Ph.D., he worked for three years with
BOC Gases in New Jersey, USA before joining Indian Institute of Technology Kanpur in 1998. He is presently the head of the chemical engineering
department and coordinator of Center for Environmental Science and Engineering at IIT Kanpur. He has published more than 60 papers in reputed
international journals and holds three patents. His research interests are in adsorption, carbon nanofibers and nanoparticles, environmental
remediation, and transport modeling.