Journal of Biotechnology & Biomaterials
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The present study investigates the effect of PH, RPM
and substrate concentration (Sweet sorghum juice)
on biological hydrogen production using cattle dung
(containing mixed culture) as the source of inoculum.
Initial PH was found to have a profound effect on
hydrogen production, while regulating the PH 6.5
through out the fermentation was found to increase
the cumulative hydrogen production significantly. At
regulated PH (6.5) and RPM (150) higher H2 production
(2800 ml/150 ml juice) was recorded. The volatile fatty
acids are always associated with hydrogen production
and the amount of VFA obtained in the present study
is 2600 mg /L. Further, maintaining the PH of medium
at 6.5 investigations were carried out to study the
effect of microbial community in cattle dung to acidic
environment using HCl at varying concentrations. It was
also found that the microbial community in cattle dung
was adapted to acidic environment obtained using 0.2
N HCl enhanced the biological hydrogen production.
However, use of HCl beyond 0.2N for adaptation of
microbial community in cattle dung exhibited an
inhibitory effect on hydrogen production.
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