alexa

GET THE APP

In Vitro Assessment Of Chrome Cytotoxicity In Human Fibroblasts | 17219
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

6th International Conference on Vaccines and Immunology

Geneva, Switzerland

3rd European Congress on Virology

Geneva, Switzerland
Google scholar citation report
Citations : 1600

Journal of Biotechnology & Biomaterials received 1600 citations as per google scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

In vitro assessment of chrome cytotoxicity in human fibroblasts

5th World Congress on Biotechnology

Maestre I, Cuesta N, Albert E, Bertazzo M, Mart?nez P and Mart?nez MA

Posters: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.028

Abstract
Around 80-85% of leather is produced using chrome, in its trivalent (III) form, as a tanning agent. For different reasons, part of this Cr (III) can be transformed into Cr (VI) in the leather itself. Cr (VI) is carcinogenic and mutagenic if inhaled, toxic if ingested and allergenic when in contact with the skin. It is estimated that up to 0.11% of the European population is allergic to Cr (VI). Although the European Commission is expected to establish this year a limit of 3 ppm for the amount of Cr VI/ kg of leather in goods intended to be in contact with skin, some chrome VI sensitised patients have developed cutaneous allergic reactions to values under this limit. However, the analytical standard test methods currently applied feature a detection limit of 3 ppm.By means of an in vitro method (cultured human fibroblasts), cytotoxicity assays were performed by exposing them to known Cr (VI) concentrations (>3 ppm) prepared in a 5.5 pH artificial sweat buffer for 24h, and then analysing cell viability by means of the MTT cytotoxicity test. Furthermore, extracts were obtained in the same buffer from chrome-tanned leather containing Cr VI, in order to determine if the cytotoxicity was attributable to Cr (VI) exclusively, or if it was modulated by the effect of another compounds present in tanned leather.
Biography
Maestre I completed her PhD degree in the field of cell biology at Miguel Hernandez University (Alicante, Spain). She has been working as a researcher in INESCOP (Footwear Technological Institute) since 2005, being part of the Biotechnology Department.
Top