In Vitro Culture Of Moringa Oleifera, Multipurpose Specie For Chile | 17294
Journal of Biotechnology & Biomaterials
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Moringa oleifera is a native crop to North India, Bangladesh, Afghanistan and Pakistan. Currently grows throughout the
tropics. Fruits, seeds and roots are used for human consumption and their stems are used for animal feed. The species
has become very important because contain 18 of the 20 amino acid that the human body required and is one of the plant
species with higher seed oil content, between 30-40%. The aim of this study was to evaluate
germination of zygotic
and to select genotypes by their
survival. For the embryo rescue, seeds were disinfected in 70%
ethanol (v/v) for 3 min and 50% sodium hypochlorite (v/v) for 20 min followed by successive rinses with sterile water. The seed
coats were removed and zigotic embryo was rescued. Embryos were transferred to Murashige and Skoog (MS) culture medium
with macronutrients diluted at 25% and cultured in both, dark and light conditions, at 25?1?C. After 14 days an 88% and 77%
of germination in dark and light conditions was obtained, respectively. These results are the first reports of
in Chile and show the ability to generate a culture protocol to enhance future nutraceutical uses in the Country.
Priscila Carteshas completed her Doctorate degree at the age of 29 years from the University of Concepci?n, Chile. She is Director at Vitroflora Austral Ltda.
managing the scientific area of the company. She has participated in more than 10 papers in reputed journals and has attended several conferences en the Forest
biotechnology area, especially in plant tissue culture issue.
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