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In the present work, three Sorghum accessions known to show different levels of salinity tolerance (viz., tolerant, moderately
tolerant and sensitive) at plant level have been used to study their in vitro response to cultures under in vitro salinity stress.
The immature inflorescences of moderately tolerant (IS. 23217 Zm. 138,) ,tolerant (IS. 23190 Zm. 90) and sensitive (IS
23253 Zm.321 ) accessions(characterized from previous studies ) collected from field grown plants were inoculated on MS
medium (containing different concentrations of NaCl and MgSO
) supplemented with 1.5 mg/l 2,4-D for callus induction and
0.25mg/l BA for regeneration. Data were collected on Frequency of callus induction, frequency of embryogenic calli, total callus
quantity, embryogenic callus quantity and regeneration frequency under different salinity concentrations. With an increase in
salinity concentration, there was decrease in all the in vitro characters. In control moderately tolerant(IS 3566) showed better
response, but in salinity treatments IS 23217 Zm.138 identified as tolerant at plant level retained its tolerant nature under in vitro
conditions also. Therefore it is suggested that, the in vitro screening using callus cultures can be applied for large scale screening
of germplasm to identify the salinity tolerant and sensitive genotypes.
Kusuma Kumari Panda has completed her PhD from Andhra University and postdoctoral studies at Indian Agricultural Research Institute, New Delhi.
She has 10 years of teaching experience and presently working as Assistant Professor at Amity University, Noida, U.P.
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