alexa

GET THE APP

Intracellular Proteome Profile Of Phanerochaete Chrysosporium During The Lapachol Biotransformation Process | 48972
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

26th World Congress on Biotechnology

Prague, Czech Republic

27th World Congress on Biotechnology

Prague, Czech Republic

27th Global Congress on Biotechnology

Paris, France

29th Asia Pacific Biotechnology Congress

Dubai, UAE
Google Scholar citation report
Citations : 1820

Journal of Biotechnology & Biomaterials received 1820 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Intracellular proteome profile of Phanerochaete chrysosporium during the lapachol biotransformation process

10th Asia-Pacific Biotech Congress

Luciana Barbosa Coitinho, Nathalia G Rosa Garzon, Flavio Emery and Hamilton Cabral

University of Sao Paulo, Brazil

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.056

Abstract
Biotransformation studies can be useful for the production of new molecules with industrial and pharmaceutical interest. The lapachol, a natural product is a naphthoquinone that presents many biological activities which have potential uses in the pharmaceutical industry. The aim of this work is to analyze the proteome profile of Phanerochaete chrysosporium during the lapachol biotransformation process. The microorganism was cultivated in 250 mL Erlenmeyer flasks containing 50 mL of soy-glucose medium and incubated on a rotary shaker at 120 rpm at 30 °C. After 24 hours, the mycelial mass was transferred to a Czapeck medium containing lapachol solution in DMSO (final concentration of 25 μg per mL) and bioprocess was continued for 24 hours. Control, without lapachol was also made. The mycelial mass was lysed, the proteins were precipitated with TCA 10% and acetone and 2D electrophoresis was performed in triplicate. The gels were analyzed by ImageLab 2D platinum v7.0 (GE) software. In addition, the proteolytic activity of intracellular proteome and secretome were determined using fluorescence resonance energy transfer (FRET) substrate. It identified 621 and 585 spots of control and lapachol respectively. Of these, we obtained 399 common to both, 222 control exclusive and 186 lapachol exclusive. Only the control secretome showed proteolytic activity. The obtained results suggest that the fungus modifies the profile of the proteins production during the lapachol biotransformation process.
Biography

Luciana Barbosa Coitinho was graduated in Pharmacy and Biochemistry and has a Master’s degree in Biotechnology. She is currently a PhD student of Pharmaceutical Sciences program at the University of Sao Paulo, Brazil.

Email: [email protected]

Peer Reviewed Journals

Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals

Clinical & Medical Journals

Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Top