Isolation And Antagonistic Ability Of Lactic Acid Bacteria From Some Indigenous Nigerian Fermented Foods | 12193
Journal of Biotechnology & Biomaterials
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This study aims to isolate lactic acid bacteria (LAB) from some Nigerian indigenous fermented foods (ogi, eko, fufu, iru) in
order to exploit their antimicrobial potential.
A total of 347 LAB strains were isolated from 150 samples of the fermented foods using standard procedures. These were
characterized as LAB based on their biochemical, morphological and physiological studies. Ogi from sorghum (sogi) had the
highest number of isolates with the isolation rate of 4.45 per sample while eba had the least with an isolation rate of 0.15. Generally
fresh fermented foods had a higher number of LAB isolates then cooked ones. The LAB strains were majorly rods (86.74%) while
only 13.27% were cocci. The predominant Lactobacillus species was L. plantarum.
Preliminary screening of the 347 isolates against 3 indicators (Bacillus subtilis, Escherichia coli and Lactobacillus casei)
organisms revealed that about sixty per cent (205) of the LAB strains exhibited antagonistic activity against at least one of the
indicator organisms, using the agar spot method. Bacillus subtilis was the best indicator as it was inhibited by 136 of the 205
LAB isolates. Only 98 isolates with inhibition zones greater than 5mm were selected for further screening. Sixty three of the 98
isolates retained the ≥5mm inhibition zones when treated with the cell free culture filterate (CFCS). Neutralisation and treatment
of the CFCS with catalase revealed that the antagonistic activity of 16 of the 63 isolates were bacteriostatic. . Forty percent of
the bacteriocidal isolates were from sorghum ogi while cooked fufu recorded the least (4%). Sensitivity test of the antibacterial
substances produced by 25 of the bacteriocidal LAB strains showed that they were inactivated by at least one proteolytic enzymes,
which indicate their protein nature.
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