alexa

GET THE APP

Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Isolation And Characterization Of Ovine Embryonic Stem Cells | 4731
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 1770

Journal of Biotechnology & Biomaterials received 1770 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Isolation and characterization of ovine embryonic stem cells

3rd World Congress on Biotechnology

P. Iyya Shankar

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Abstract
Embryonic stem (ES) cells have become very important resources in basic medical researches. ES cells are derived from explanted culture of the inner cell mass (ICM) of blastocyst stage embryos and has definite explicative potential and differentiate into derivatives of all three germ layers. Initially invitro fertilized embryos were cultured using TCM 199 +20% Fetal bovine serum + L?Glutamin 2mMol and Penicillin/Streptomycin 100mg/100IU/ml for 3 days. These cell lines were generated by removing ICM from preimplantation embryos using 0.75% pronase. Es cells are grown on a layer of mitotically inactivated primary mouse embryonic fibroblast (MEF) feeder layer to promote growth and prevent differentiation using mitomycin ? c, primary embryonic fibroblast were isolated from 13 to 14 days old fetuses. The cells were disaggregated and trypsinized every 3 days until the appearance of the colonies of ES cells. The colony positive cells were fixed and stained for alkaline phosphatase. The ES cells were cultured in suspension state for 5 days; at the same time Leukaemia Inhibitory Factor (LIF) was removed from media to form embryoid bodies (EBs). Undifferentiated cells were seen as intensely small cells attached together compactly with high nucleus/cytoplasm (N/C) ratio. The cells of colonies tend to differentiate by separation from each other and became larger and diffused on substrate by attaching to dish. The positive alkaline phosphatase cells were seen in typical morphology of ES colonies. The EBs cells were seen in culture after 5 days in suspension and began to spontaneously differentiate into various types of cells such as nerve and hematopoitic lineages.
Biography
P. Iyya Shankar completed her Post graduation in M.Sc Biotechnology from University of Madras in the year 2007. He is currently working as Senior Executive ? cell culture department in Life cell International Private ltd, Chennai, Tamil Nadu, previously working as Senior Research Fellow in Department of Animal Biotechnology, MVC, Tanuvas, Chennai ? 600053, Tamil Nadu, India.
https://milanobet.fun/ https://mobilbahis.fun/ https://oleybet.fun/ https://onbahis.fun/ https://padisahbet.fun/ https://piabetgiris.xyz/ https://pusulabet.fun/ https://pulibet.fun/ https://restbet.fun/ https://satbet.fun/ https://1xbetx.fun/ https://asyabahisx.xyz/
Top