Isolation Of Cellulose Degrading Microorganisms From Cow Rumen | 34004
Journal of Biotechnology & Biomaterials
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The application of fossil fuels results serious environmental problems moreover these energy sources are getting exhausted.
These facts inspire the resarchers and developing labs to find alternative solutions. The cellulose/hemicellulose-based
technologies can provide the solution. Cellulose is the most abundant organic compound on the Earth and it is found many
places, e.g. in the cell wall of plants between lignin and hemicellulose. The most important component of hemicellulose is
xylan. High amount of cellulose/hemicellulose is produced by industry, agriculture, etc. Many microorganisms are able to
degrade these biomaterials under aerobic or anaerobic conditions with complex enzyme systems. Breakdown of cellulose/
hemicellulose by cellulases and xylanases results monosaccharides an/or secondary metabolits that can be further fermented
by other microorganisms. During the progress several alternative energy carriers e.g. biodiesel, bioethanol, H2, etc. can be
produced. Our goal was to isolate cellulose and xylan degrading microorganism(s) from cow rumen content under aerobic
conditions. We isolated two strains which showed good cellulase and xylanase activity on minimal salts agar plate supplemented
with microcrystalline cellulose or xylan as carbon source. The activity of enzymes were determined by Congo-red probe then
strains were identified by 16S rDNA-based method. The physiological and biochemical characteristics of the isolates were
determined, as well.
Árpád Szilágyi has completed his MSc degree. He studied at University of Szeged, Faculty of Science and Informatics as biologist (Molecular-, Immun- and Microbiology specialization). He started his PhD programme at Doctoral School of Environmental Sciences in 2013 and his supervisor is Dr. Gábor Rákhely.