Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Isolation Of Staphylococcus Sps. From Mobile Phones And In-vitro Study Of Mollugo Cerviana Leaf Extract On Staphylococcus Sps. | 4757
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Isolation of Staphylococcus sps. from mobile phones and in-vitro study of Mollugo cerviana leaf extract on Staphylococcus sps.

3rd World Congress on Biotechnology

G. Mahesh, Sudhamalla B, Amooru DG and Subramanyam R

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Human serum albumin (HSA) is an important plasma protein responsible for the binding and transport of many endogenous and exogenous substances including hormones, fatty acids and foreign molecules such as drugs. Most of the drugs can bind to HSA and be transported to target locations of the body. For this study, Trimethoxy flavone (TMF) is used which is a naturally occurring flavone isolated from Andrographisviscosula and used in the treatment of dyspepsia, influenza, malaria, respiratory functions and as a astringent and antidote for poisonous stings of some insects. In order to see the binding, conformation and binding sites of the HSA-TMF complexation we used fluorescence emission, mass spectrometry, circular dichroism and molecular docking parameters. The fluorescence emission was quenched and the binding constant of TMF with HSA was found to be K TMF =1.0 + 0.01 x 103 M -1 , which corresponds to -5.4 kcal M -1 of free energy. Micro-TOF Q results showed a mass increase of from 65,117 Da (free HSA) to 66,689 Da (HSA +Drug), indicating the strong binding of TMF with HSA resulting in decrease of fluorescence. The HSA conformation was altered upon binding of TMF to HSA with decrease α-helix and an increase in β-sheets and random coils suggesting partial unfolding of protein secondary structure. Molecular docking experiments found that TMF binds strongly with HSA at IIIA domain of hydrophobic pocket with hydrogen bond and hydrophobic interactions. Among which two hydrogen bonds are formed between O (19) of TMF to Arg 410, Tyr 411 and another one from O (7) of TMF to Asn 391, with bond distance of 2.1?, 3.6? and 2.6?, respectively. In view of the evidence presented, it is imperative to assign a greater role of HSA?s as a carrier molecule for many drugs to understand the interactions of HSA with TMF will be pivotal in the design of new TMF-inspired drugs.
G. Mahesh completed his M.Sc in Biochemistry in the year 2005 from University of Hyderabad, Hyderabad, Andhra Pradesh, after pursuing his master?s degree, he joined as a JRF under Dr. S. Rajagopal, Reader, Department of Plant sciences. Now he is pursuing his Ph.D under the same supervisor in the field of Biochemistry. In the past four years he has published 5 papers in reputed international journals (Plos one, Journal of photobiology and photochemistry B, The Journal of physical Chemistry B, Journal of Molecular modeling).