Journal of Biotechnology & Biomaterials
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Monochoria vaginalis is a native aquatic medicinal
plant of Kerala. In this study Populations of
Monochoria vaginalis from different geographical
locations was compared by using RAPD marker. The
RAPDs provide a useful tool for assessing genetic
diversity of rare, endemic species and for resolving
relationships among populations. For the above study ten
plants were collected from five different geographical
locations. Fourteen decamer oligonucleotide primers
were used to amplify the genomic DNA isolated from
fresh leaves of the collected plants. Ten primers initially
screened displayed RAPD profiles with polymorphic
bands and many of these bands varied in molecular
weight and intensity. The number of polymorphic bands
scored per primer ranged from 2 to 8. Dendrogram
were constructed using UPGMA algorithm based on the
similarity index values. The present study shows that
RAPD analysis is useful for genetic diversity analysis.
Genetic characterization not only provides database
for genetic biodiversity but also is a necessity for the
protection of native species.
I Smitha Thomas.K., a research scholar doing research under the guidance of Dr.Linu
Mathew in school of biosciences, Mahatma Gandhi University, Kottayam, India. My area of
research is plant molecular biology.
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