Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Reach Us +44 3308186230
GET THE APP
Label-free Proteomics Of The Fetal Pancreas Identifies Deficits In The Peroxisome In Rats With Intrauterine Growth Restriction | 109620
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Aim: The objective of the present study was to identify differentially expressed proteins (DEPs) in the pancreas of a fetus
with intrauterine growth restriction (IUGR) and to investigate the molecular mechanisms leading to adulthood diabetes
in IUGR.
Methods: The IUGR rat model was induced by maternal protein malnutrition. The fetal pancreas was collected at
embryonic day 20 (E20). Protein was extracted, pooled and subjected to label-free quantitative proteomics analysis.
Bioinformatics analysis (GO and IPA) was performed to define the pathways and networks associated with DEPs. LC-MS
results were confirmed by western blotting and/or quantitative PCR (q-PCR).The principal parameters of oxidative stresssuperoxide
dismutase (SOD) were determined in blood samples of fetal rats.
Results: A total of 57 DEPs (27 upregulated, 30 downregulated) were identified with a 1.5-fold change threshold and a
p-value≤0.05 between the IUGR and control pancreas. Bioinformatics analysis revealed that these proteins play important
roles in peroxisome biogenesis and fission, fatty acid beta oxidation (FAO), mitotic cell cycle and histone modification.
The peroxin Pex14 was downregulated in the IUGR pancreas as confirmed by western blotting and q-PCR.Pmp70, a
peroxisomal membrane protein involved in the transport of fatty acids, was upregulated. Hsd17b4 and Acox1/2, which
catalyze different steps of peroxisomal FAO, were dysregulated. SOD plasma concentrations in the IUGR fetus were higher
than those in the control, suggesting partial compensation for oxidative stress.
Conclusion: The present study identified DEPs in the fetal pancreas of IUGR rats by proteomics analysis. Down regulation
of pancreas peroxins and dysregulation of enzymes involved in peroxisomal FAO may impair the biogenesis and function
of the peroxisome and may underlie the development of T2 diabetes mellitus in adult IUGR rats. The present data provide
new insight into the role of the peroxisome in the development of the pancreas and may be valuable in furthering our
understanding of the pathogenesis of IUGR-induced diabetes.
Biography
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
+44 3308186230
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 