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This work reports on the isolation of Lactic acid bacteria (LAB) from non-broiler chicken, other than commonly useed
broiler chicken. Lactic acid bacteria (LAB) were isolated from the ceca, intestine, gizzard and bile of Malaysian non-broiler
chicken ?ayam kampung?. Following the incubation on MRS agar plates, 56 LAB isolates were collected. Based on agar disk
diffusion method, 5 out of 56 isolates; L3, L4, L5, L7 and L7 show inhibition against the indicator bacteria Methylene-resistant
Staphylococcus aureus MRSA. All of the five isolates show typical characteristics of Enterococcus family which are Gram positive
coccus morphology, non-spore forming, catalase negative, producing acid from glucose and able to grow on 4, 6 and 10% NaCl.
Ability to produce inhibitory proteins using agar disk diffusion method were tested using three phase partitioning (TPP) purified
fraction employing t-butanol and ammonium sulphate. The purified fraction produced zones of inhibition (approx. 16-18 mm in
diameter) on indicator organism Staphylococcus aureus MRSA. The purified fraction contained putative low molecular weight
proteins of about 8-10 kDa in size, as determined by SDS-PAGE. These putative proteins are stable upon heat treatments and
inactivated by trypsin. The partial rDNA sequences from the 5 isolates showed to be at least 98% similarity with Enterococcus
family. Isolates L3, L4, and L5 belonged to the E. faecum. Meanwhile, L7 and L10 belonged to E. faecalis and E. mundtii. These
strains were exclusively isolated from non-broiler chicken; and they produce putative inhibitory proteins or bacteriocins against
Staphylococcus aureus MRSA are useful as probiotic for chicken.
Tengku Haziyamin Tengku Abdul Hamid has completed his PhD in 2009 at University Putra Malaysia in the area of enzyme technology. He is
currently the Deputy Dean Academic at Faculty of Science, International Islamic University Malaysia Kuantan campus.
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