Mass Spectrometry Identification Of Pathogens In Various Sample Solutions Using Functionalized Magnetic Iron Oxide (Fe3O4) Nanoparticles | 4671
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Mass spectrometry identification of pathogens in various sample solutions using functionalized magnetic iron oxide (Fe3O4) nanoparticles

3rd World Congress on Biotechnology

P. Muralidhar Reddy and Yen-Peng Ho

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Nanotechnology has triggered a revolution in many important areas in molecular biology, biotechnology and medicine, especially in the detection and manipulation of biological species at the molecular and cellular levels. With the advent of nanotechnology in biology and medicine, the need for conjugation of nanoparticles (NPs) to biomolecules has grown. In this study, magnetic and functionalized magnetic iron oxide nanoparticles were synthesized and used as affinity probes to capture Gram-positive/negative bacteria. The morphology and properties of these magnetic NPs were examined by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and zeta potential measurements. We have investigated the interaction between functionalized magnetic nanoparticles and bacteria. A variety of electrostatic and covalent linking chemistries have been reported. Furthermore, the magnetic nanoparticles with various functionalities including Fe3O4, poly acrylic acid (PAA), TiO2and SiO2 were employed to concentrate target bacteria from sample solutions. The trapped bacteria were then analyzed by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS).
P. Muralidhar Reddy received B.Sc., M.Sc., and Ph.D. (chemistry) degree from Kakatiya University, Warangal, India. Currently, he is working as a postdoctoral fellow with Prof. Yen-Peng Ho at National Dong Hwa University (NDHU), Taiwan. He has published more than 50 papers in reputed journals and presented research articles in various conferences. Dr. Reddy received two gold medals, one at the BS level and the other at the MS level. He also received best paper presentation award (Ranked First) in ICNN-2011 and the most popular student award in 1999. He is a member of Indian Council of Chemists (ICC) and Taiwan Society for Mass spectrometry (TSMS).