Journal of Biotechnology & Biomaterials
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The recent progress in biotechnology has been made it possible to the combination of both protein and whole cell together
to explore a wide range of applications. In this talk, the display of a series of enzymes such as xylose dehydrogenase, glucose
dehydrogenase and organophosphorus hydrolase on the surface of Escherichia coli and glucose oxidase on yeast surface for the
construction of whole-cell biocatalysts are demonstrated. The characterization of the fusion protein by proteinase accessibility,
Western blot, and enzyme activity assays is performed. The optimal temperature and pH of the enzyme displayed cells are
discussed. Finally, the sensitive and selective electrochemical biosensing of monosaccharides or organophosphates and
assembly of biofuel cell on basis of the microbial surface displaying enzymes are highlighted. The enzyme-displayed bacterial
cell can not only be used directly without further enzyme extraction and purification, but also it improves the stability of the
enzyme. Therefore, microbial whole-cell would be engineered to develop novel approaches, which could be more efficient and
cost-effective in comparison with traditional analytical techniques.
Aihua Liu is a Full Professor of Qingdao Institute of Bioenergy & Bioprocess, Chinese Academy of Sciences, where he leads the Laboratory of Biosensing. He
received his PhD degree from Tohoku University, Japan (2004) with major in bioelectrochemistry and biosensing. Followed he worked in the National Institute
of Advanced Industrial Science & Technology (AIST), Japan under the Japanese Society for the Promotion of Sciences (JSPS) fellowship (2004-2006). After
that he moved to the US and conducted his postdoc research in Michigan State University (2006-2007), University of Oklahoma (2007-2009), and University of
Texas at Arlington (2009-2010). His current research interests cover Microbial Surface Display, Biosensor, Biochips, and Biofuel Cells. He is the managing editor
to Frontiers in Bioscience. He has published over 40 peer-reviewed papers in leading journals including AngewandteChemie International Edition, Advanced
Materials, Analytical Chemistry, Biosensors & Bioelectronics and Journal of Molecular Biology.
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