Modulation Of Calcium Oxalate Crystals Morphology By Aqueous Extract Of Tribulus Terrestris | 39088
Journal of Biotechnology & Biomaterials
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Tribulus terrestris has an ancient tradition in folk and in Ayurveda as a diuretic, antiseptic and anti-inflammatory agent, it is
also a common constituent of various marketed antiurolithiatic herbal formulations like Uriflow, Uritone, Cystone, Neeri
and Culdisol, etc. To investigate the effect of aqueous extract (AE) of Tribulus terrestris of different extraction parameters i.e.,
AE1 (temp. 23.50°C, time 19.50 hours and S:L 12:00), AE2 (temp. 4.16°C, time 19.50 hours and S:L 12:00), AE3 (temp. 35°C,
time 36 hours and S:L 12:00) and AE4 (temp. 35°C, time 3 hours and S:L 20:00) on calcium oxalate crystals (COM) formation,
crystals size and morphology of crystals, polarization microscopic imaging technique was used. To investigate the effect of test
sample, 50 μl of CaCl2 (4.25 mM), 50 μl of Na2C2O4 (0.75 mM) and 50 μl of test samples were mixed and incubated at 37°C
for 45 minutes. Crystal morphology was examined in five randomly selected fields at 40× magnification from different fields
under an upright microscope (Olympus Corporation, Japan). Microscopic analysis showed that the aqueous extract AE1 and
AE2 reduces the number of crystals, shine of crystals and also showed distortion in morphology of COM crystals as compared
to AE3 and AE4. In conclusion, AE1 and AE2 found more effective then AE3 and AE4.
Jyoti Kaushik has completed her MSc Biotechnology from Lovely Professional University, Punjab. She is currently pursuing her PhD in Biotechnology and working as SRF in DBT sponsored project under the guidance of Dr. Chanderdeep Tandon from Amity Institute of Biotechnology, Amity University, India.