Journal of Biotechnology & Biomaterials
Open Access

Abstract

Based on the research results reported by Escribano et al. (2004 and 2008) for cherimoya (A. cherimola), a total of 20 microsatellite molecular markers (SSR), reported as the most transferability in the genus Annona and Rollinia, were employed to evaluate genetic diversity through cross amplification, in a in situ collection of Colombian Andina and Caribe Region. The study looked at the stage of DNA extraction, PCR amplification and PAGE visualization, followed by statistical analysis to inter and intra population. The results allowed the preliminary development of DNA extraction protocols, PCR and PAGE to Anonáceas. Four SSR were transferable in six species and one interspecific hybrid, and the most representative species (A. squamosa, A. reticulata and A. cherimola) were evaluated through six, seven and seven SSR, respectively. The analysis intrapoblacional (geographic regions) denotes genetic diversity (observed heterozygosity), while in interpopulation analysis reveals that in the collections of A. squamosa and A. reticulata, heterozygote deficit occurs and most of the variation occurs within populations a priori (between individuals within populations), detecting a low degree of genetic differentiation. The calculation of genetic distances and graphing through a neighbor joining topology, allows for A. squamosa identify that there is a greater number of shared alleles among accessions of Cundinamarca and other accessions tested, whereas in A. reticulata this presents among accessions from Santander and Cundinamarca and Casanare.

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