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Molecular Analysis Of Genetic Diversity: An In Situ Collection Of Annonaceae?s Species By Using Microsatellite Markers | 17263
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
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Based on the research results reported by Escribano et al. (2004 and 2008) for cherimoya (A. cherimola), a total of 20
microsatellite molecular markers (SSR), reported as the most transferability in the genus Annona and Rollinia, were
employed to evaluate genetic diversity through cross amplification, in a
in situ
collection of Colombian Andina and Caribe
Region. The study looked at the stage of DNA extraction, PCR amplification and PAGE visualization, followed by statistical
analysis to inter and intra population. The results allowed the preliminary development of DNA extraction protocols, PCR and
PAGE to Anon?ceas. Four SSR were transferable in six species and one interspecific hybrid, and the most representative species
(A. squamosa, A. reticulata and A. cherimola) were evaluated through six, seven and seven SSR, respectively. The analysis
intrapoblacional (geographic regions) denotes genetic diversity (observed heterozygosity), while in interpopulation analysis
reveals that in the collections of A. squamosa and A. reticulata, heterozygote deficit occurs and most of the variation occurs
within populations a priori (between individuals within populations), detecting a low degree of genetic differentiation. The
calculation of genetic distances and graphing through a neighbor joining topology, allows for A. squamosa identify that there
is a greater number of shared alleles among accessions of Cundinamarca and other accessions tested, whereas in A. reticulata
this presents among accessions from Santander and Cundinamarca and Casanare.
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