Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Molecular Characterization And Structure Analysis Of A Putative Tumor Suppressor QM Homologue From The Cabbage Butterfly Pieris Rapae | 4892
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Molecular characterization and structure analysis of a putative tumor suppressor QM homologue from the cabbage butterfly Pieris rapae

3rd World Congress on Biotechnology

Bharat Bhusan Patnaik, Bo Mi Park, Seung Han Oh, Dong Hyun Kim and Yeon Soo Han

AcceptedAbstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.021

The highly-conserved tumor suppressor, QM-like gene homologues have been cloned and characterized from various organisms including vertebrates such as mammals and fish, invertebrates such as insects (D. melanogaster) and shrimp (P. japonicus), plants, yeast and protozoan parasites. The gene encodes a ribosomal protein L10 and has been implicated to function during cell growth, cell differentiation, apoptosis and immunological defence mechanisms. The study describes the molecular characterization and structure analysis of a putative tumor suppressor, QM-like gene (designated as PrQM) found in a normalized whole tissue cDNA library of the cabbage butterfly, Pieris rapae, considered to be a serious agricultural pest in the world. The sequence comprised of 660-bp open reading frame and encoded a protein of 219 amino acids corresponding to 26 kDa protein with predicted ribosomal protein L10a/L10e domain. PrQM amino acid sequence showed high levels of conservation at their N-terminal regions with putative QM homologues from other insects. The apparent difference in functional modules in insects can therefore be attributed to the less conserved C-terminus. The cDNA corresponding to PrQM was thereafter cloned into pET28 (+) vector and expressed in E.coli BL21 cells, and the recombinant protein purified by His-tag affinity chromatography. The recombinant PrQM was detected as a single band of expected molecular weight of 29 kDa in SDS-PAGE. Polyclonal PrQM antibody was generated in Swiss-albino rats and western blotting studies recognized a polypeptide with a molecular weight of 26 kDa, relating to the endogenous PrQM protein, consistent with the theoretical molecular size. The anti-PrQM antibody was ultimately used to understand sub-cellular localization of the PrQM in infection models of Pieris rapae larvae.
Bharat Bhusan Patnaik has completed his doctorate from University of Madras in the year 2005 and is the recipient of International Scholar Exchange Fellowship by Korea Foundation for Advanced Studies for the year 2011-12. He has also served as UGC-NRCBS Visiting Fellow to Madurai Kamaraj University, Madurai and received the Indian Academies of Science Fellowship for Teachers to Visva-Bharathi University, Santiniketan. He has published quality papers in International and National journals and is a reviewer to Elsevier and academic journals.