Molecular Characterization Of Trichoderma Isolates As Biological Control | 17056
Journal of Biotechnology & Biomaterials
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Twelve isolates of Trichoderma were isolated from soil rhizosphere of plantation crops represent different geographic areas
in Egypt. These isolates were morphologically identified and the genetic relatedness of these isolates was analyzed by
PCR-based Random Amplified Polymorphic DNA (RAPD) using 10 random primers. RAPD profiles showed high genetic
diversity among the isolates with the formation of four distinct clusters. The Antagonistic abilities of Trichoderma isolates were
evaluated against Rhizoctonia solani and Pythium aphnidermatum as soil borne plant pathogens in dual culture plates. The
results of antagonistic tests showed in general that these isolates are more active in inhibition growth of P. aphnidermatum than
R. solani and the isolate of Trichoderma T8 was the most efficient isolate in inhibition growth of P. aphnidermatum and of R.
solani of 78% and 73% respectively. However, the Trichoderma isolates T7 and T6 showed the lowest inhibition activity against
P. aphnidermatum and R. solani respectively.
Khalid Abdel-lateif is a Lecturer of Genetics, Menoufia University, Egypt. He has completed his PhD degree in Biology of Plants (IRD, University of Montpellier 2,
France). He has more than five research publications and attended more than ten international conferences.
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