Journal of Biotechnology & Biomaterials
Open Access

Abstract

Antibiotic resistance and associated genes are ubiquitous creating a call of dare throughout the world. As recently recognized extended-spectrum β-lactamases (ESBLs) have been found the most extensive and threatening mechanism of antibiotic resistance, both in clinical and non-clinical settings. These ESBL enzymes cause resistance to oxyimino cephalosporins, such as cefotaxime, ceftazidime and ceftriaxone and to monobactams, such as aztreonam, resulting in resistance to these drugs. ESBLs are by and large mutants of classical bla-TEM and bla-SHV genes. Many ESBLs emerged among clinical pathogens like CTXM- type enzymes have established to be the most successful in terms of promiscuity and diffusion in different epidemiological settings, where they have largely replaced and outnumbered other types of ESBLs. The name CTX imitates the potent hydrolytic activity of these β-lactamases against cefotaxime. Till now, 158 variants of CTX-M enzymes have been identified and can be classified by amino acid sequences into subgroups: CTX-M1, CTX-M2, CTX-M8, CTX-M9 and CTX-M25. In our study, we evaluated the presence of different CTX-M subgroups in each E. coli CTX-M positive isolate from UTI patients, by PCRdenaturing gradient gel electrophoresis (PCR-DGGE) of the CTX-M gene. For this, we had designed a single pair of primers for all subgroups of CTX-M genes. More than one band was observed in E.coli isolate from UTI sample run on DGGE, indicating the presence of different variants of CTX-M gene in E. coli isolate.

Biography

Asif Anas is currently pursuing his PhD from Department of Bioscience, Jamia Millia Islamia, India. He has completed his MSc in Enzyme and Fermentation Technology from Department of Biochemistry, Aligarh Muslim University, Aligarh. He has presented one international and four sequence submissions in NCBI. The focus of his current study is on drug resistance in E. coli.

Email: aasifanassiddiqui@gmail.com