Journal of Biotechnology & Biomaterials
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Tissue engineering off ers a contemporary paradigm
for periodontal tissue regeneration in which periodontal
ligament stem cells and eff ective cellular growth factors
are very essential. Th e objective of this study was, for the
fi rst time, to investigate the capabilities and advantages
of an herbal medicine, Morinda citrifolia leaf aqueous
extract for induction of osteogenic diff erentiation and
matrix mineralization of human periodontal ligament
Methods: Th e optimal biocompatible dose of
Morinda citrifolia leaf aqueous extract was determined
by a cell viability assay. Th e human periodontal ligament
stem cells were cultured in complete medium, ascorbic
acid together with -glycerophosphate or Morinda
citrifolia leaf aqueous extract. Th e osteogenic cell
diff erentiation marker, alkaline phosphatase activity,
was assayed using a biochemical analysis. Matrix
mineralization was examined aft er 6 weeks by calcium
staining, scanning electron microscopy and energy
dispersive x-ray analysis.
Results: Morinda citrifolia leaf aqueous extract
signifi cantly increased alkaline phosphatase activity in
either complete medium or ascorbic acid together with
-glycerophosphate. Nodules containing mineralized
matrices were formed only when the cells were cultured
in the presence of Morinda citrifolia leaf aqueous extract.
Th ese nodules were positively stained with alizarin red
S and were rich in calcium and phosphorus as revealed
with energy dispersive x-ray analysis.
Conclusion: Th ese data indicate that Morinda
citrifolia leaf extract can promote osteogenic
diff erentiation as well as matrix mineralization in
human periodontal ligament stem cells and suggest that
Morinda citrifolia leaf may have therapeutic benefi ts in
bone and periodontal tissue regeneration.
Dr. Kanitsak Boonanantanasarn DDS, PhD is a Diplomat of the American Board of Periodontology. Recently, he has joined Dr. Steven Gill’s laboratory at the Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry as a postdoctoral fellow and has an adjunct clinical position at the SUNY at Buffalo, School of Dental Medicine. He is a faculty member in the Department of Anatomy, Faculty of Dentistry, Mahidol University, Thailand. His research interests include growth factors induce tissue engineering and oral microbiota, oral carcinogenesis and stem cell. Additionally, Dr. Boonanantanasarn also discovers natural growth factors from tropical herbs which initiates stem cell differentiation for periodontal tissue engineering. Moreover, in Thailand, he is a pioneer in the screening and development of tropical herbs which demonstrate anticancer properties in oral cancers. He has applied for several patents for these herbs as an adjunctive chemotherapy and inducers for tissue engineering.
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