Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Nanomedicine Based Delivery To Systems For The Treatment Of Degenerative Arthritis | 4805
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Nanomedicine based delivery to systems for the treatment of degenerative arthritis

3rd World Congress on Biotechnology

Jagat R. Kanwar, Rasika M. Samarasinghe and Rupinder K. Kanwar

Posters: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.020

Osteoarthritis (OA) and Rheumatoid arthritis (RA) are the most common forms of joint diseases affecting over 250 million people worldwide. As with most auto-immune and inflammatory diseases, the severity of arthritis is aggregated by the activation of inflammatory responses mediated by biochemical agents such as cytokines, chemokines, prostaglandins, growth factors, proteases and nitric oxide (NO). Current treatments results in severe systemic side effects such as cardiovascular failure, kidney dysfunction, diabetes, gastrointestinal bleeding and osteoporosis. Here we demonstrate the activity of a naturally derived protein purified from cow?s milk as an alternative treatment option for OA. Two different forms of the protein, metal saturated (P M+ ) and unsaturated (P M- ) were used. Furthermore in order to increase efficient delivery and increase bioavailability, a novel non- toxic, biodegradable polymeric nanoformulation was utilised to encapsulate the bioactive proteins. Characterization techniques such as dynamic light scattering and scanning electron microscopy revealed particle size range to be between 180nm-220nm and have a spherical morphology. Differential scanning calorimetry demonstrated an enhanced thermal stability of protein loaded nanoparticles as compared to the void (unloaded) nanoparticles. Fourier transform infrared spectroscopy analysis confirmed the interactions of protein with nanoparticles through the enhanced chemical bonding and X-ray diffraction patterns revealed no change in the crystalline structure between protein loaded and void nanoparticles. The cellular and molecular efficacy of these nanoparticles was examined on osteoarthritic chondrocytes (cartilage cells) to evaluate its anti-inflammatory and regenerative activity. Nanoparticles were observed to be non-toxic to cells and were able to internalise within 2 hours as determined by confocal microscopy. The non-toxicity was further confirmed with cytotoxic and apoptotic assays which showed no significant change in cell integrity even after treatment with increasing concentrations. The anti-inflammatory activity of nanoparticles, determined through various assays, showed significant inhibition of agents reported to initiated cartilage degradation and inflammation. Chondrocytes treated for 24 hours with P M+ nanoparticles significantly down- regulated the production of nitric oxide and prostaglandins as compared to P M- and void particles. Gene expression analysed with real-time PCR revealed the down regulation of key inflammatory mediators such as matrix metalloproteinases (MMP)-1, MMP-2, MMP-3, MMP-9, MMP-13, MMP-14, MMP-15, nitric oxide synthase 2, cytokines interleukin-1β and tumour necrosis factor-α. Moreover, P M+ nanoparticles were able to increase expression of aggrecan and type II collagen. Protein expressions of MMP-3, MMP-9 and NOS2 were down regulated with P M+ treatments. Ex-vivo loop assay testing the absorbance of nanoparticles in different sections of the mouse intestine revealed an increased absorbance of P M- and P M+ nanoparticles within 2 hours in the jejunum and ileum whereas void nanoparticles only showed a significant absorbance after 4 hours. Furthermore, P M- showed an increased absorbance in the jejunum and P M+ within the ileum. These findings collectively demonstrate the regenerative and anti-inflammatory activity of natural bioactives loaded nanoparticles and its potential use as a nanotherapeutic for arthritis and other rheumatic related diseases.