Nephroprotective Efficacy Of Apocynin Against Hyperoxaluria Induced Nephrolithiasis In Male Wistar Rats | 40637
Journal of Biotechnology & Biomaterials
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Hyperoxaluria is an imperative risk factor for calcium oxalate stone formation in nephrolithiasis. The in vitro and in vivo
investigations indicated that oxidative stress plays a significant role in the development of kidney stone. NADPH oxidase
is considered as a major source of ROS in hyperoxaluric conditions. Apocynin, a NADPH oxidase inhibitor, is a natural nontoxic
compound isolated from a medicinal plant, Picrorhiza kurroa. It prevents activation of NADPH oxidase by blocking
the assembly of cytosolic units with the membrane complex. Hence the present study was designed to investigate the effect
of apocynin on ethylene glycol induced hyperoxaluria in male Wistar rats. Four groups were designed with 6 animals each.
Control rats were given normal saline intraperitonealy. EG group rats received 0.4% ethylene glycol and 1% ammonium chloride
in drinking water for 9 days to induce hyperoxaluria. Apocynin group rats received apocynin alone (200 mg/kg body weight,
i.p. per day) for 9 days. EG+Apocynin group rats received 0.4% ethylene glycol and 1% ammonium chloride in drinking water
along with apocynin (200 mg/kg body weight, i.p. per day) for 9 days. Urine was collected on 9th day and rats were sacrificed
on day 10. Their kidneys were processed for localization of crystals and various other biochemical analyses. Results indicated
significant reduction in the oxidative stress and improvement in the renal dysfunction in apocynin treated hyperoxaluric rats.
In conclusion, apocynin presented itself as a safe and effective remedy in combating hyperoxaluria induced nephrolithiasis.
Minu Sharma is a Senior Research Fellow pursuing her PhD from Panjab University, Chandigarh. She has three publications in reputed journals, one original research paper and two review papers.