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Neutralizing Antibody Responses Flowing Vaccination And Correlation With Commercial Serologic Tests | 109881

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Neutralizing antibody responses flowing vaccination and correlation with commercial serologic tests

5th International Conference on Vaccines and Immunology

Amal Souiri, Sanaa Lemriss and Saad El Kabbaj

Laboratory of Research and Medical Analysis of Gendarmerie Royale, Morocco

ScientificTracks Abstracts: J Infect Dis Ther

Abstract
SARS-CoV-2 has caused a global pandemic with millions infected and numerous fatalities. A better understanding of the relationship between functionally neutralizing antibodies and binding antibodies is needed in order to address protective immunity postinfection or vaccination. In this study, we investigate the humoral immune response and the seroprevalence of neutralizing antibodies following vaccination with adenovirus-based vector in 177 serum samples. Microneutralization (MN) assay was used as a reference method to assess whether neutralizing antibody titers correlated with a positive signal in two commercially available serological tests, a rapid lateral flow immune-chromatographic assay (LFIA) and an Enzyme-linked fluorescence assay (ELFA). Neutralizing antibodies were detected in majority of serum samples (84%). COVID-19 convalescents individuals had higher antibody titers and higher neutralizing activity. Spearman correlation coefficients between serological and neutralization results ranged from 0.8 to 0,9 indicating moderate to strong correlation between commercial immunoassays test results (LFIA and ELFA) and virus neutralization. Keywords: SARS-CoV-2; Serology; Neutralizing Antibody; COVID-19; Vaccination
Biography

Amal Souiri is currently head of Cell Culture Laboratory, Department of Biosafety PCL3, Laboratory of Research and Medical Analysis of Gendarmerie Royale in Morocco. She earned her PhD in Virology, Immunology & Molecular biology from the Faculty of Science, Mohammed V University in Morocco.

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