Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
New Developments In Yarrowia Lipolytica Yeast Expression System And Its Application To Genetic Engineering Of Heterologous Proteins | 4603
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
More than 100 heterologous proteins from various phylogenetic origins have been successfully produced in Yarrowia lipolytica yeast expression system, which offers reliable performances in glycosylation, folding and secretion of complex proteins. We present here a short review of the major tools developed in this yeast, with focus on vectors for targeted monocopy integration
(YLEX expression kit, Yeastern Biotech Co, Taiwan), a system particularly adapted to genetic engineering of heterologous proteins.
In order to examplify the various applications of Yarrowia expression system, a number of current collaborative works on whole-cell biocatalysis, genetic engineering of secreted enzymes, and surface-display of heterologous proteins (arming yeasts) will be briefly resumed. Some applications, like high throughput screening, require improving Yarrowia transformation methods for higher efficiency, without sacrificing the high percentage of correctly targeted integrations. For that purpose, we have developed a sensor system for homologous integration, based on E. coli lacZ gene expression, which allowed us to evaluate some chemicals increasing transformation efficiency for maintenance of a high percentage of correct integration. Dithiothreitol was found to be not only more efficient than dimethyl-sulfoxide in increasing transformation efficiency, but also compatible with homologous
integration, which was not the case of the latter that promoted out-of-site integration. An optimized transformation protocol using dithiothreitol has been developed, which ensures very high transformation efficiency.
Catherine Madzak has been a student at Ecole Normale Sup?rieure, one of the main French governmental schools of higher education. She
obtained her Ph.D. in Microbiology at the Cancer Institute of Villejuif. She is now a senior research associate at INRA, the French National Institute
for Agricultural Research. Her main research subject is to develop an expression system for heterologous proteins in the yeast Yarrowia lipolytica,
with particular emphasis on genetic engineering of enzymes. She is author of more than 40 publications in international journals, five book chapters,
and inventor of a patent on a recombinant promoter.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals