Journal of Biotechnology & Biomaterials
Open Access

Abstract

The objective of this study was to look into molecular mechanism of puberty and generate useful information for the molecular breeding to shorten the timing of puberty of goat. KiSS-1/GPR54 system is universally considered as a key regulator and a catalyst for the puberty onset in mammals. Literature concerning KiSS-1 and goat production is rare. Thus partial sequence of KiSS-1 gene was amplified and sequenced for identification of Single nucleotide polymorphism (SNP) in a panel of 9 Indian goat breeds differing in precocity and prolificacy. A primer pair was designed according to goat sequence (GU142847.1) with expected product length of 472bp covering exon 2 along with its upstream and downstream regions. Comparison of obtained sequences resulted in identification of two SNPs (T950C, T1125C). The sites of mutation were numbered according to goat KiSS-1sequence (GU142847.1). There were no polymorphisms in the exon 2 of indigenous goat as reported sites were located in upstream (Intron1) and downstream (Intron1) of exon 2. Wild allele was predominant in indigenous goats as compared to mutant allele for both SNPs (T950C T=0.92, C=0.08 and T1125C T=0.96, C=0.04). For SNP in intron1 (T950C) all the three genotypes (TT, TC and CC) were identified in Osmanabadi breed while two genotypes (TT and TC) were detected in Beetal, Barbari and Ganjam while wild type was observed in Black Bengal, Malabari, Jakhrana, Sangamneri and Sirohi and for SNP in intron 2 (T1125C) all the breeds except Jakhrana (TT and TC) presented wild type allele. Although two novel variants do not concern the coding region, it might influence the stability of mRNA, can affect the mechanism of deadenylation and degradation and consequently, the amount of proteins produced. Further studies are necessary for association of polymorphisms with goat sexual precocity in goats.

Biography

Rekha Sharma has completed her PhD from National Dairy Research Institute (Deemed University) Karnal, India in 1998. She is Senior Scientist at NBAGR, a premier organization involved in characterization, conservation and utilization of Animal Genetic resources. She has published more than 25 papers in reputed journals on molecular characterization of AnGR with neutral and functional markers.