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The objective of this study was to look into molecular mechanism of puberty and generate useful information for the molecular
breeding to shorten the timing of puberty of goat. KiSS-1/GPR54 system is universally considered as a key regulator and
a catalyst for the puberty onset in mammals. Literature concerning KiSS-1 and goat production is rare. Thus partial sequence
of KiSS-1 gene was amplified and sequenced for identification of Single nucleotide polymorphism (SNP) in a panel of 9 Indian
goat breeds differing in precocity and prolificacy. A primer pair was designed according to goat sequence (GU142847.1) with
expected product length of 472bp covering exon 2 along with its upstream and downstream regions. Comparison of obtained
sequences resulted in identification of two SNPs (T950C, T1125C). The sites of mutation were numbered according to goat
KiSS-1sequence (GU142847.1). There were no polymorphisms in the exon 2 of indigenous goat as reported sites were located
in upstream (Intron1) and downstream (Intron1) of exon 2. Wild allele was predominant in indigenous goats as compared to
mutant allele for both SNPs (T950C T=0.92, C=0.08 and T1125C T=0.96, C=0.04). For SNP in intron1 (T950C) all the three
genotypes (TT, TC and CC) were identified in Osmanabadi breed while two genotypes (TT and TC) were detected in Beetal,
Barbari and Ganjam while wild type was observed in Black Bengal, Malabari, Jakhrana, Sangamneri and Sirohi and for SNP in
intron 2 (T1125C) all the breeds except Jakhrana (TT and TC) presented wild type allele. Although two novel variants do not
concern the coding region, it might influence the stability of mRNA, can affect the mechanism of deadenylation and degradation
and consequently, the amount of proteins produced. Further studies are necessary for association of polymorphisms with goat
sexual precocity in goats.
Rekha Sharma has completed her PhD from National Dairy Research Institute (Deemed University) Karnal, India in 1998. She is Senior Scientist at
NBAGR, a premier organization involved in characterization, conservation and utilization of Animal Genetic resources. She has published more than
25 papers in reputed journals on molecular characterization of AnGR with neutral and functional markers.
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