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Novel extracellular lipases from Trichosporon asahii MSR54: Implications in cell physiology and biotechnology
6th World Congress on Biotechnology
Rani Gupta
University of Delhi, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Three novel lipases TALipA, TALipB and TALipC from opportunistic pathogen Trichosporon asahii MSR54 were isolated, sequenced and heterologously expressed in E.coli and Pichia pastoris X33. Both glycosylated and non-glycosylated proteins were characterized. These lipases are biotechnologically important because all the three lipases are enantioselective. Out of the three, TALipC has an asprich domain and shows enantioinversion in different solvents. It shows Mg activation and calcium inhibition. The role of these lipases was studied in growth and biofilm formation by knock-down strategy. The RNAi silencing and agrobacterium mediated transformation for developing knock-down strains of T. asahii was standardized in our laboratory for the first time. The lipase knock-down strains lost the property of yeast to fungal transition and predominantly retained the yeast stage. Detailed structural and functional comparisons of these lipases will be highlighted.Biography
Rani Gupta has carried out her PhD program (1983-1988) at Botany Department, Delhi University in Microbial Physiology, Biochemistry and Ecology. Since then she has been working at the Department of Microbiology University of Delhi South Campus (UDSC). Her group has been working on industrial enzymes like protease, gamma-glutamyl transpeptidase, lipase, amylase and chitinase from the past 30 years.
Email: ranigupta15@yahoo.com
