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In recent years, many studies on the genetics of prolificacy in small ruminants have highlighted the importance of BMPR1B gene
in affecting ovulation rate and litter size through different mechanisms. Most of the research on BMPR1B gene has revolved
around screening sheep and goats for the presence of FecB mutation (A746G) that is associated with hyperprolific phenotype
in many breeds around the world. In the present study, in addition to screening Indian goats for the FecB (Booroola) mutation,
an attempt was also made to detect polymorphism in the promoter of BMPR1B gene. Eight indigenous goat breeds (Barbari,
Beetal, Black Bengal, Malabari, Jhakrana, Osmanabadi, Sirohi and Ganjam) differing in prolificacy and geographic distribution
were employed for polymorphism scanning. Representative samples were collected from the breeding tract of respective breeds
and DNA was isolated using Phenol-Chloroform method. Primers for detecting FecB mutation were taken from the literature
whereas heterologous primers for promoter region were designed from the sequence of Bos taurus (ENSBTAT00000002690)
available in Ensemble database. BMPR1B promoter was amplified in two fragments and a 1032 bp length contig was generated
using SeqMan program of Lasergene software. Sequence alignments and comparisons were carried out using MegAlign program
(DNASTAR Inc.) which revealed that FecB mutation was not present in all the breeds investigated. Hundred variations were
observed between cattle and goat promoter sequence which include 32 transitions, 13 transversions, 50 deletions and 5 insertions.
Two novel SNPs T (-242) C and G (-623) A were identified in the promoter of indigenous goat breeds. The variations and
polymorphisms did not change or affect any transcription binding sites as revealed by MATCH software.
Sonika Ahlawat is working as scientist at National Bureau of Animal Genetic Resources, Karnal, India.
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