One Step Purification Of Intracellular L-asparaginase From E. Carotovora MTCC 1428 And Its Application | 4698
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
L-asparaginase is a chemotherapeutic enzyme that catalyzes the conversion of L-asparagine to L-aspartate and ammonia. The
important application of the L-asparaginase enzyme is in the treatment of acute lymphoblastic leukemia, Hodgkin disease,
acute myelocytic leukemia, chronic lymphocytic leukemia, lymphosarcoma treatment, reticulosarcoma and melanosarcoma.
L-asparaginase is widely distributed among prokaryotes and eukaryotes and since the large amount of this enzyme drug has been
needed for pharmacological and clinical tests, microbial sources are found to be best for the bulk production of L-asparaginase.
In the present study, the L-asparaginase from Erwinia carotovora MTCC 1428 was purified and used for the killing of Hep-2C cell
line. Sonication of the resting cells was carried out to release the intracellular L-asparaginase and cell free extract was subjected
to acid precipitation. Sulphopropyl Sephadex was used for further purification of enzyme. The 24% yield of the L-asparaginase
was obtained after single step purification. The specific activity of the purified enzyme was found to be 0.37 IU/mg and the
electrophoresis results suggest that the L-asparaginase of E. carotovora MTCC 1428 exists in the form of dimmer of dimmers.
Moreover, the purified L-asparaginase from E. carotovora MTCC 1428 devoid of glutaminase activity. The L-asparaginase purified
from E. carotovora MTCC 1428 showed better in vitro toxicity on Hep-2C cell lines (84% survival) in comparison to commercial
L-asparaginase preparation (90% survival) obtained from E. coli.
Sarita Devi has completed her PhD in the year 2012 at the age of 29 years from Himachal Pradesh University. She has published many papers in
the journals on international repute.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals