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Ophthalmic Disorder Management With Triphala | 8500
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Ophthalmic disorder management with Triphala

World Congress on Biotechnology

Dolly Singh, S.S.Sawhney*, R.M.Painuli*

ScientificTracks Abstracts: J Biotechnol Biomaterial

DOI: 10.4172/2155-952X.1000001

Abstract
The steroid-based eye drops with great side effects prompted us to go for the plant-based preparation for the cause in reference. The triphala-based phytosignatures extractable in different solvents of different polar index with minimal side effects were our natural choice. This study showed that the extracts of triphala in various solvents like Ethyl Acetate, Acetone, Methanol and Water were laced with different blend of phytosignatures, possessing the mitigating potential(s) for oxidative stress and bacterial pathogenecitythe main factors responsible of all types of diseases including eye infection(s). The results of Minimal Inhibitory Concentration were further used to check the biocompatibility of the extracted biosignatures with Rabbit Corneal Epithelial (SIRC) cell lines. The drug was prepared by dissolving the lyophilised triphala extracts in PBS of pH 7 made in millipore water. The proliferation (MTT) assay revealed the conclusive action of the in-built phytosignatures as the growth enhancer and promoter of the SIRC in-vitro, extendable to in-vivo application. The cell morphology and cell viability was further evaluated by staining the cell nuclei using DAPI and Hoechst stains respectively. The dose-dependent use of these ?Eye Drops? may be recommended, as the side effects with the triphala appear to be minimal. The formulation is cost-effective, accessible and economical, which could yield comparable results. This is probably the first reporting on the ocular managing properties of triphala.
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