alexa Optimization Of Catalase Activity By Rhodotorula Glutinis Using Experimental Design | 63585
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Joint Event on 15th World Congress on Biotechnology And Biotech Industries Meet and 2nd International Conference on Enzymology and Molecular Biology

Ayse Ezgi Unlu and Serpil Takac
Ankara University, Turkey
Posters & Accepted Abstracts: J Biotechnol Biomater
DOI: 10.4172/2155-952X.C1.071
Abstract
Rhodotorula glutinis is a pigmented, salt tolerant yeast and also gains attention due to its oleogenic property. It has high capacity to produce antioxidant molecules such as carotenoids. However, limited research has been conducted on the synthesis of other antioxidant molecules such as catalase (CAT) enzyme. CAT is a heme protein that is present in animal cells, bacteria and plants and it decomposes hydrogen peroxide to water and oxygen (Eqn. (1)). It is widely used in various industrial areas such as textile, food and cosmetics. 2H2O2→O2+2H2O (1) The aim of this study is to investigate the parameters that provided the optimum conditions for high CAT activity by Rhodotorula glutinis, and to search for the potential utilization of glycerol as a carbon source for high CAT activity, which is a by-product of biodiesel plants. For this aim, central composite design (Design Expert 7.0.0) including 20 runs with 6 central points was performed and temperature (TºC) (10.6-32.4ºC), initial medium pH (pH) (3.99-6.0) and glycerol concentration (Gly, gL-1) (9.77-60.23) were selected as factors to be optimized for the response, CAT activity, according to the previous findings of the research group. The following second order model (Eqn. (2)) was proposed: CAT (U)=-4.36106+1.34381(pH)+ 0.051575(T)+ 0.028907(Gly)-0.13551(pH)2 -3.78071x10-4(Gly)2 (2) The model was found to be statistically significant (R2=0.94, R2adj=0.92, model F value 40.95, lack of fit value 2.21). The most effective factor on CAT activity was found as temperature (p<0.0001). The response surface graphics are presented at Fig. 1. Fig. 1a was obtained when Gly was 37.03 mg mL-1. According to the figure, the highest CAT activity was obtained at high T and low pH values. Similarly, Fig. 1b showed that activity increased with increasing T however, medium values of Gly provided higher activity values, maximum at 37.5 mg mL-1. According to Fig. 1c, the highest CAT activity values were obtained at the medium values of both pH and Gly. As a result of the experiments, it was found that determination of the maximum predicted response required a shift of the experimental region to higher temperature values.
Biography

Ayse Ezgi Unlu has expertise in enzymes, enzymatic reactions, fermentation, protein synthesis, proteomics, enzymatic biopolymers and green solvents. The synthesis of Naproxen, a member of NSAIDs, was the subject of her Master’s thesis by using commercial lipase subjected to various pre-treatment strategies that enhanced the activity. Investigation of different parameters on the production of lipase by Candida rugosa and also proteomic analysis of the isoenzymes was another subject of her interest. She has done her Post-doctoral research on the synthesis of flavonoids using green solvents.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7