Journal of Biotechnology & Biomaterials
Open Access

Abstract

Cellulases are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganisms that grown over cellulosic matters. Bacterial cellulases possess more advantages when compared to the cellulases from other sources. Cellulase producing bacteria was isolated from Cow dung. The organism was identified using 16 SrRNA sequencing and BLAST search. One hundred different strains of Bacteria were isolated from cow dunk in Sohag governorate. Isolates were tested for their ability to produce extracellular Cellulases and hemicellulases. Of all these isolates Bacillus sp. that had the highest potential for Celluolytic and hemicelluolytic activity was chosen. From various morphological and physiological and properties, this isolate was found to belong to Bacillus sp. Physiological studies were conducted to determine the optimum cultural conditions for maximal cellulases and hemicellulases formation by B. cereus LAZ 518. Shaking condition at 150 rpm is favorable for the production of cellulases and hemicellulases from corn cobs. The highest enzyme yield was obtained at the second day of incubation (after 48 hours) at 50 �?°C. however cellulases and hemicellulases formation occurred at a broad range of pH values, with maximal formation of B. cereus LAZ 518 cellulases and hemicellulases at pH 7.0 when corn cobs was used as sole carbon source, respectively Carboxymethyl cellulose (CMC) was found to be a good inducer for cellulases and hemicellulases formation with CMC concentrations of 1.0% (w/v) in case of corn cobs. High level of enzyme formation was obtained with the addition of yeast extract as a nitrogen source and at a concentration of 0.1% (v/v) Tween-80 as an addition to medium containing corn cobs increases enzymes production.

Biography

Email:

abu.gharbia@yahoo.com