Optimization Of Process Variables For Biodegradation Of Phenol By Pseudomonas Putida (NCIM 2102) Using Response Surface Methodology | 4833
Journal of Biotechnology & Biomaterials
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Numerous industrial applications raised chromium to a very important economic element. At the same time, with the
development of its uses, the adverse effects of chromium compounds in human health were being investigated. Both acute
and chronic toxicity of chromium are mainly caused by hexavalent compounds. The present study deals with the removal of
hexavalent chromium compound using bioremediation by an acclimated mixed culture developed from activated sludge. The
acclimatization of culture was carried out for a period of 6 days by decreasing the amount of glucose from 1000 mg/l to 0 mg/l
with a decrement of 200 mg/l every day and increasing Cr (VI) concentration accordingly by the same amount. At the end of
the period, the biomass concentration obtained for final amount of pure Cr (VI) was 0.0187 g/50 ml of solution. The effect of
one of the major operating parameters, contact time on bioremediation of Cr (VI) by acclimated mixed culture was also studied
in a batch mode. This study had been conducted for an initial Cr (VI) concentration ranging from 20-80 mg/L and pH ranging
from 2-10. Increase in initial Cr (VI) concentration from 20 mg/L to 80 mg/L showed increase in Cr (VI) uptake. Experimental
investigation also showed that percent removal of Cr (VI) exceeding 94% at the initial concentration of 80 mg/L of Cr (VI) and pH 7.
Subhajit Majumder, Lecturer of Chemical Engineering at Birla Institute of Technology and Science (BITS), Pilani, India, received his B.E. degree
from University of Pune, India and M.E. degree from Chemical Engineering Department of BITS, Pilani in 2007. Mr. Majumder?s main professional
activity has been in the area of biological based separation techniques and various novel separation methods. He has co-authored theoretical and
experimental technical papers pertaining to his field of research. He is a member of the American Chemical Society and Asia-Pacific Chemical,
Biological & Environmental Engineering Society.
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