Optimization Of TGE (Transient Gene Expression) With Peptone Feeding Strategy For Recombinant Protein Production In Mammalian Cell Cultures | 12168
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

6th International Conference on Vaccines and Immunology

Geneva, Switzerland

3rd European Congress on Virology

Geneva, Switzerland
Google scholar citation report
Citations : 1600

Journal of Biotechnology & Biomaterials received 1600 citations as per google scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Optimization of TGE (Transient Gene Expression) with peptone feeding strategy for recombinant protein production in mammalian cell cultures

4th World Congress on Biotechnology

Fatemeh Davam

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.023

Today, almost 60-70% of recombinant proteins are successfully produced in CHO cells. The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. Transient expression of the protein (Transient Gene Expression) TGE is the preferred way to express recombinant proteins in a short timeframe and at a scale suitable for pre-clinical testing. In the present study, the effect of optimization of transfection condition in a serum free medium CD DG44 in a Shaking incubator with CHO DG44 cells in suspended condition was evaluated . A range of values for DNA, PEI and Cell concentration were studied to find the optimal values regarding transfection efficiency. The results showed that the optimized values were 1.5 μg/106 cells of trasfection reagent (PEI), 0.5*106 cells for starting cell densities and 2 μg/106 cells of DNA with 28.71, 38.68 and 52.8% transfection efficiency respectively. Furthermore, based on these TGE optimized condition effect of feeding with 6 different Soy and Caseine peptones on transfection efficiency was evaluated The results of peptone feeding with peptones Caseine Tryptone N1, Soy Peptone A2Sc and Soy peptone E110 showed 59.88.%, 58.28% and 68.66% transfection efficiency with 15.44, 12.35 and 28.55% increase compared to previous optimized process. These data shows that the appropriate feeding strategy is capable of increasing transfection efficiency and is a promising approach for optimizing TGE process.