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Optimization Of The Operating Parameters For Beta Galactosidase Production On Cheese Whey | 17113
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Optimization of the operating parameters for beta galactosidase production on cheese whey

5th World Congress on Biotechnology

Khaled Boudjema, Abellellah Amarache, Yamina Benmiri, Fethia Fazouane-Naimi and Amina Hellal

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.027

Abstract
Beta-galactosidase belong to the group of enzymes able to cleave β linked galactose residues from various compounds and is commonly used to cleave lactose into galactose and glucose. The objective of this study was to enhance the sweet cheese whey for beta galactosidase production by using an Algerian strain of lactic acid bacteria, Streptococcus thermophilus . Firstly, different physicochemical analysis of cheese whey were carried out includingash, protein, fat and lactose. Secondly, the operating parameterssuch as temperature, pH, inoculum size and incubation period were optimized. Finally, the production of beta galactosidase was carried out in the optimized conditions. The results of physicochemical analysis of sweet cheese whey have shown that it has an adequate quality given it high nutrient materials such as: Lactose (37.24 g/l), ash (8.32 g/l), protein (3.11 g /l) and low fat amount (0.4 g / l). Moreover, the results of the optimisation of beta galactosidase production indicatedthatthe optimum values of temperature, pH, inoculum size and incubation periodwerefound to be 40?C, 6.5, 10% (v/v) and 18 h respectively. Under these optimales conditions, the production of β-galactosidase has presented a maximum specific activity of 314, 91 IU/g.
Biography
Khaled Boudjema is preparing his PhD at university of Boumerdes, Algeria. He is an assistant-teacher in department of Biology and researcher in research laboratory of food technology at the same university.
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