alexa Optimization Of The Synthesis Of Galacto-oligosaccharides (GOS) From Lactose By Two Commercial β-galactosidases
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

5th World Congress on Biotechnology
June 25-27, 2014 Valencia Conference Centre, Valencia, Spain

Isabel M Gonz?lez Delgado, Mar?a-Jos? L?pez-Mu?oz, Gabriel Morales and Yolanda Segura
Posters: J Biotechnol Biomater
DOI: 10.4172/2155-952X.S1.028
Abstract
The demand for foods with demonstrable health benefits has recently increased due to the general awareness on nutrition. Galacto-oligosaccharides (GOS) are currently an interesting example of this kind of functional foods. They are polymers of glucose and galactose (Galn-Glc) which are non-digestible and act as prebiotics by stimulating the proliferation of lactic acid bacteria and bifidobacteria in the human intestine. In addition, they present low calorific content being excellent food and drink sweeteners. The enzyme β-galactosidase (EC 3.2.1.23) can synthesize GOS from lactose (transgalactosylation reaction), but also hydrolyze GOS and lactose (hydrolysis reaction). Even though both reactions take place simultaneously, the desired transgalactosylation can be favoured at high lactose concentration, elevated temperature and low water content. Moreover, the GOS composition of the reaction mixture can be influenced by a number of factors, including initial lactose concentration, enzyme source, pH and temperature. In this work two commercial enzymes have been used to evaluate the β-galactosidase activity: Lactozym Pure 6500 L, a beta-galactosidase from Kluyveromyceslactis , and Pectinex Ultra SP-L, a poligaracturonase from Aspergillusaculeatus . Both enzymes were first characterized in order to evaluate their hydrolysis activity at different pH (from 4.5 to 7.5) and temperature (from 40 to 60?C) following the well-known oNPG method. Then, the experimental conditions for the GOS synthesis such as temperature, pH, lactose and enzyme concentrations have been studied, aiming to optimize the production of tri- or higher oligosaccharides.
Biography
Isabel M Gonz?lez Delgado is presently undertaking her second year of her PhD in the area of Food Biotechnology in the group of Chemical and Engineering Technology of the Rey Juan Carlos University of Madrid (Spain). She gained her degree in Biotechnology at the Pablo de Olavide University. Afterwards, she undertook a Master related to Rare Diseases. During these years she has collaborated in several research projects in the area of molecular biology, genetic engineering, biocomputing and molecular modelling.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords