Optimized Extraction Of Microalgae?s Metabolites: A Crucial Step In High-throughput Screening Programs Dedicated To Phytoplankton Chemodiversity | 17126
Journal of Biotechnology & Biomaterials
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With the recent development of state-of-the-art technologies (
hyphenated MS techniques) and methodologies
dereplication), the scientific community is interested in the exploration of poorly chemically studied bioresources.
The high diversity of interacting phytoplankton species suggests an important and highly diverse chemical repertoire
isoprenoids, toxins, polysaccharides, PUFAs, oxylipins, phycobiliproteins) which may inspire applications in health,
nutrition and biotechnology. Biosynthesis of these metabolites is strongly dependent upon their environment/culture
conditions which may be investigated using OMICS approaches. In microalgae, a major bottleneck isthe difficulty in extracting
deeply inaccessible molecules, an important issue that demands adapted solutions prior to considering High-Throughput
Screening (HTS). Bioactive minority metabolites may pass unnoticed on spectra and thus require special attention. The
extraction of metabolites may prove difficult due to the presence of highly resistant cell walls (
of exopolysaccharidic secretions surrounding the cell membrane (
). The Mix Mill process (vibrating
microbeads) which gave excellent extraction yields without chemical alteration of the analytes) and is fully compatible with
HPLC and LC-MS analysis was optimised. Being accurate, simple to operate, rapid, safe and preserving sensitive molecules,
makes the Mix Mill process suitable for the screening of microalgalchemodiversity. This methodology was applied in the
Photomer, and currently in OCEANOMICs and OCEANCHArCoT programs, all being dedicated to the identification of
new marine metabolites with high added value. Finally, this methodology represents a significant improvement in the field of
OMICS studies from microalgae, as it provides the most representative estimate of their exploitable chemical diversity.
Serive B after a marine oriented academic cursus (PhD from University of Nantes in 2012) and professional experiences in various companies and laboratories,
has recent interests focused on marine pigments which exhibit novel activities in photodynamic therapies (Photomer program) which may also find applications
in oceanography. He was recently awarded a Marie Curie International Outgoing Fellowship with OCEANCHArCoT program (OCEAN Chemodiversity against
Cell cycle Targets). In recognition to his unfailing commitment in marine Bioresources, he was also awarded the 2006 Bleustein Blanchet Foundation prize for
entrepreneurship of young scientists.
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